ATTO 589H
Optical Properties |
|||
λabs |
589 |
nm |
|
εmax |
1.2×105 |
M-1 cm-1 |
|
λfl |
620 |
nm |
|
ηfl |
85 |
% |
|
τfl |
3.9 |
ns |
|
CF260 =ε260/εmax |
0.36 |
|
|
CF280 =ε280/εmax |
0.52 |
|
ATTO 589H is a fluorescent marker from the class of rhodamines. As a hydrophilic alternative to ATTO Rho101, the dye shows very good water solubility, significantly reduced aggregation behavior and a low tendency to non-specific binding. ATTO 589H has a strong absorption, very high fluorescence quantum yield and a high thermal and photochemical stability. The dye is therefore perfectly suited for use in single molecule detection and high-resolution microscopy (SIM, STED, etc.). ATTO 589H is an anionic dye. After coupling to a substrate, it carries a single negative charge. Efficient excitation of fluorescence occurs in the 570 - 600 nm range. ATTO 589H is an isomer mixture of two dyes with practically identical absorption and fluorescence properties. The dye is also available in pure isomer form on request.