ATTO 584H
|
Optical Properties |
|||
|
λabs |
584 |
nm |
|
|
εmax |
1.2×105 |
M-1 cm-1 |
|
|
λfl |
607 |
nm |
|
|
ηfl |
80 |
% |
|
|
τfl |
3.5 |
ns |
|
|
CF260 =ε260/εmax |
0.28 |
|
|
|
CF280 =ε280/εmax |
0.38 |
|
|
ATTO 584H is a fluorescent marker that belongs to the class of rhodamines. As a hydrophilic alternative to ATTO Rho101, the dye shows very good water solubility and a significantly reduced tendency to non-specific binding. ATTO 584H has a strong absorption, very high fluorescence quantum yield and a high thermal and photochemical stability. The dye is therefore perfectly suited for use in single molecule detection and high-resolution microscopy (SIM, STED, etc.). ATTO 584H is an anionic dye. After coupling to a substrate, it carries a single negative charge. Efficient excitation of fluorescence occurs in the 570 - 600 nm range.