ATTO 576H
Optical Properties |
|||
λabs |
576 |
nm |
|
εmax |
1.2×105 |
M-1 cm-1 |
|
λfl |
598 |
nm |
|
ηfl |
90 |
% |
|
τfl |
4.0 |
ns |
|
CF260 =ε260/εmax |
0.26 |
|
|
CF280 =ε280/εmax |
0.10 |
|
ATTO 576H is a fluorescent marker out of the class of rhodamines. As a hydrophilic alternative to ATTO Rho12, the dye shows very good water solubility and a significantly reduced tendency to non-specific binding. ATTO 576H has a strong absorption, very high fluorescence quantum yield and a high thermal and photochemical stability. The dye is therefore perfectly suited for use in single molecule detection and high-resolution microscopy (SIM, STED, etc.). ATTO 576H is an anionic dye. After coupling to a substrate, it carries a net electrical charge of -1. ATTO 576H is a mixture of three isomers with practically identical absorption and fluorescence properties. Efficient excitation of fluorescence occurs in the 550 - 585 nm range.