ATTO 343H
Optical Properties |
|||
λabs |
343 |
nm |
|
εmax |
1.9×104 |
M-1 cm-1 |
|
λfl |
441 |
nm |
|
ηfl |
90 |
% |
|
τfl |
4.8 |
ns |
|
CF260 =ε260/εmax |
0.19 |
|
|
CF280 =ε280/εmax |
0.18 |
|
ATTO 343H is a hydrophilic short wavelength fluorescent marker with a coumarin structure. The characteristic properties of this dye include high fluorescence quantum yield, large Stokes-shift, good photostability and low molecular weight. ATTO 343H is very water soluble and the fluorescence efficiency after coupling to biomolecules, such as a protein, is only slightly reduced even at higher degrees of labeling (DOL). ATTO 343H is an anionic dye. After conjugation to a biomolecule, the dye carries a single negative charge. Efficient excitation of fluorescence occurs in the 330 - 365 nm range.