ATTO 647N








Optische Eigenschaften |
||||
λabs |
646 |
nm |
||
εmax |
1,5×105 |
M-1 cm-1 |
||
λfl |
664 |
nm |
||
ηfl |
65 |
% |
||
τfl |
3,5 |
ns |
||
CF260 = ε260/εmax |
0,04 |
|
||
CF280 = ε280/εmax |
0,03 |
|
ATTO 647N zählt im roten Spektralbereich zu einer neuen – speziell von ATTO-TEC entwickelten – Generation von Fluoreszenzmarkern. Zu den charakteristischen Eigenschaften des Farbstoffs zählen starke Absorption, sehr hohe Fluoreszenzquantenausbeute, hohe thermische und photochemische Stabilität sowie außergewöhnlich hohe Stabilität gegenüber atmosphärischem Ozon. ATTO 647N ist somit besonders geeignet im Bereich der Einzelmoleküldetektion sowie zur hochauflösenden Mikroskopie (SIM, STED, etc.).
Absorption und Fluoreszenz sind im pH-Bereich von 2 - 11 pH-unabhängig. Der Farbstoff ist mäßig hydrophil. ATTO 647N ist ein kationischer Farbstoff. Nach der Kopplung an ein Substrat ist der Farbstoff einfach positiv geladen. ATTO 647N ist ein Gemisch aus zwei Isomeren mit praktisch identischen Absorptions- und Fluoreszenzeigenschaften. Eine effiziente Anregung der Fluoreszenz erfolgt im Bereich 625 - 660 nm. Für den Farbstoff geeignete Anregungslichtquellen sind neben dem HeNe-Laser (633 nm) der Krypton-Ionen-Laser (647 nm) oder gängige Diodenlaser mit Emission bei 650 nm.
Produktdatenblätter
- ATTO 647N Produktinformation
- NHS-Ester
- Maleimid
- Iodacetamid
- Phalloidin
- Amin
- Azid/Alkin
- Streptavidin
- Tetrazin (MeTet)
Absorptions- und Emissionsspektrum (ASCII)
- ATTO 647N Absorption (.txt)
- ATTO 647N Emission (.txt)
Absorptions- und Emissionsspektrum (Grafik)
- ATTO 647N Absorption/Fluoreszenz (.jpg)
Sicherheitsdatenblätter
- ATTO 647N Carboxy MSDS
- ATTO 647N NHS-Ester MSDS
- ATTO 647N Maleimid MSDS
- ATTO 647N Streptavidin MSDS
- ATTO 647N Biotin MSDS
- ATTO 647N Phalloidin MSDS
- ATTO 647N Amin MSDS
- ATTO 647N Azid MSDS
- ATTO 647N Iodacetamid MSDS
- ATTO 647N Hydrazid MSDS
- ATTO 647N Alkin MSDS
- ATTO 647N Cadaverin MSDS
- ATTO 647N MeTet MSDS
Ausgewählte Literatur zu ATTO 647N:
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