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ATTO 643

 
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ATTO 643
ATTO 643

Optical Properties

λabs

643

nm

εmax

1.5×105

l / mol cm

λfl

665

nm

ηfl

62

%

τfl

3.5

ns

CF260 = ε260max

0.05

 

CF280 = ε280max

0.04

 

ATTO 643 is a new fluorescent label related to the well known ATTO 647N. Characteristic features of the label are strong absorption and high thermal and photo-stability. The dye is very hydrophilic, hence it shows excellent water solubility and significantly reduced tendency for unspecific binding. ATTO 643 exhibits an extraordinary high fluorescence quantum yield. Contrary to many common fluorescent labels this is the case even when the dye is coupled to proteins, e.g. antibodies, streptavidin etc. The dye is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. The label is also highly recommended for oligonucleotide labeling, flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more.
In common with most ATTO-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications. ATTO 643 is an anionoic dye. After coupling to a substrate the dye carries a net electrical charge of -1. For details of coupling see our recommended labeling procedure (PDF). As supplied ATTO 643 consists of a mixture of three isomers with practically identical absorption and fluorescence properties. The fluorescence is excited most efficiently in the range 625 - 660 nm.

Product Sheets

- ATTO 643 product information

- NHS-ester

Streptavidin


Material Safety Data Sheets


- ATTO 643 carboxy MSDS

- ATTO 643 NHS-ester MSDS

- ATTO 643 maleimide MSDS

- ATTO 643 streptavidin MSDS

- ATTO 643 phalloidin MSDS

- ATTO 643 azide MSDS



Absorption and Emission Spectrum (ASCII)


- ATTO 643 absorption (.txt)

- ATTO 643 emission (.txt)

Modification:
carboxy (1 mg) - AD 643-21
180,00 EUR
carboxy (5 mg) - AD 643-25
720,00 EUR
NHS-ester (1 mg) - AD 643-31
220,00 EUR
NHS-ester (5 mg) - AD 643-35
880,00 EUR
maleimide (1 mg) - AD 643-41
280,00 EUR
maleimide (5 mg) - AD 643-45
1.120,00 EUR
streptavidin (1 mg) - AD 643-61
240,00 EUR
streptavidin (5 mg) - AD 643-65
960,00 EUR
phalloidin (10 nmol) - AD 643-81
280,00 EUR
phalloidin (20 nmol) - AD 643-82
490,00 EUR
azide (1 mg) - AD 643-101
300,00 EUR
azide (5 mg) - AD 643-105
1.200,00 EUR
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