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ATTO 647N

 
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ATTO 647N
ATTO 647N

Optical Properties

λabs

646

nm

εmax

1.5×105

l / mol cm

λfl

664

nm

ηfl

65

%

τfl

3.5

ns

CF260 = ε260max

0.04

 

CF280 = ε280max

0.03

 

ATTO 647N belongs to a new generation of fluorescent labels for the red spectral region. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photo-stability, and exceptionally high stability towards atmospheric ozone. Thus ATTO 647N is highly suitable for single-molecule detection applications and high-resolution microscopy such as PALM, dSTORM, STED etc. Additionally the dye highly qualifies to be applied in flow cytometry (FACS), fluorescence in-situ hybridization (FISH) and many more.
In common with most ATTO-labels, absorption and fluorescence are independent of pH, at least in the range of pH 2 to 11, used in typical applications. The dye is moderately hydrophilic. ATTO 647N is a cationic dye. After coupling to a substrate the dye carries a net electrical charge of +1. For details of coupling see our recommended labeling procedure (PDF). As supplied ATTO 647N consists of a mixture of two isomers with practically identical absorption and fluorescence properties. The fluorescence is excited most efficiently in the range 625 - 660 nm. A suitable excitation source is the 647 nm line of the Krypton-Ion laser or a diode-laser emitting at 650 nm.

Literature: ATTO 647N

Product Sheets

- ATTO 647N product information

- NHS-ester

Streptavidin


Material Safety Data Sheets


- ATTO 647N carboxy MSDS

- ATTO 647N NHS-ester MSDS

- ATTO 647N maleimide MSDS

- ATTO 647N streptavidin MSDS

- ATTO 647N biotin MSDS

- ATTO 647N phalloidin MSDS

- ATTO 647N amine MSDS

- ATTO 647N azide MSDS

- ATTO 647N iodoacetamide MSDS

- ATTO 647N alkyne MSDS


Absorption and Emission Spectrum (ASCII)


- ATTO 647N absorption (.txt)

- ATTO 647N emission (.txt)

Modification:
carboxy (1 mg) - AD 647N-21
154,00 EUR
carboxy (5 mg) - AD 647N-25
616,00 EUR
NHS-ester (1 mg) - AD 647N-31
188,00 EUR
NHS-ester (5 mg) - AD 647N-35
752,00 EUR
maleimide (1 mg) - AD 647N-41
240,00 EUR
maleimide (5 mg) - AD 647N-45
960,00 EUR
streptavidin (1 mg) - AD 647N-61
200,00 EUR
streptavidin (5 mg) - AD 647N-65
800,00 EUR
biotin (1 mg) - AD 647N-71
196,00 EUR
biotin (5 mg) - AD 647N-75
784,00 EUR
phalloidin (10 nmol) - AD 647N-81
240,00 EUR
phalloidin (20 nmol) - AD 647N-82
420,00 EUR
amine (1 mg) - AD 647N-91
250,00 EUR
amine (5 mg) - AD 647N-95
1.000,00 EUR
azide (1 mg) - AD 647N-101
250,00 EUR
azide (5 mg) - AD 647N-105
1.000,00 EUR
iodoacetamide (1 mg) - AD 647N-111
250,00 EUR
iodoacetamide (5 mg) - AD 647N-115
1.000,00 EUR
alkyne (1 mg) - AD 647N-141
250,00 EUR
alkyne (5 mg) - AD 647N-145
1.000,00 EUR
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