Infos

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Data Table of ATTO Fluorescent Dyes and Quenchers:
Dye
MW , g/mol
Acid; NHS; Mal.
Structure
Spectra
lAbs , nm
eMax , l/mol cm
 CF260
CF280
lFl , nm
hFl , %
tFl , ns
343; 440; 465
390
2.4×104
 0.52
0.08
479
90
3.8
401; 498; 523
436
4.5×104
 0.27
0.23
484
90
3.5
396; 493; 518
453
7.5×104
 1.12
0.54
508
55
2.2
804; 981; 1067
patent pending
501
9.0×104
 0.25
0.10
523
80
3.2
452; 549; 574
495
8.0×104
 0.57
0.39
527
45
2.4
467; 564; 589
516
1.1×105
 0.40
0.40
538
90
3.8
765; 1081;1063
patent pending
532
1.15×105
 0.22
0.11
553
90
3.8
659; 756; 781
patent pending
542
1.05×105
 0.22
0.24
---
---
---
694; 791; 816
patent pending
554
1.2×105
 0.24
0.12
576
80
3.2
611; 708; 733
563
1.2×105
 0.34
0.16
592
90
3.4
795; 892; 917
patent pending
586
1.1×105
 0.36
0.13
---
---
---
691; 788; 813
594
1.2×105
 0.42
0.44
624
80
3.7
1137; 1389;1358
patent pending
601
1.2×105
 0.26
0.51
627
85
3.5
491; 588; 613
615
1.5×105
 0.02
0.05
634
70
3.3
494; 592; 617
611
1.0×105
 0.05
0.07
681
35
2.5
791; 888; 913
patent pending
615
1.15×105
 0.35
0.57
---
---
---
612; 709; 734
patent pending
619
1.2×105
 0.05
0.07
643
50
2.9
652; 749; 774
patent pending
629
1.3×105
 0.05
0.06
657
64
3.2
531; 628; 653
635
1.2×105
 0.13
0.10
659
25
1.9
552; 838; 716
patent pending
635
1.2×105
 0.05
0.02
659
25
1.9
592; 811; 832
patent pending
645
1.2×105
 0.08
0.04
669
20
2.3
746; 843; 868
patent pending
644
1.5×105
 0.06
0.05
669
65
3.4
634; 887; 812
patent pending
663
1.25×105
 0.24
0.08
684
30
1.9
631; 828;1024
patent pending
680
1.25×105
 0.30
0.17
700
30
1.8
575; 837; 971
patent pending
700
1.2×105
 0.26
0.41
719
25
1.5
516; 613; 638
patent pending
729
1.2×105
 0.10
0.08
752
10
0.5
568; 665; 690
patent pending
740
1.2×105
 0.11
0.10
764
10
0.6

 

MW:
Molecular weight, including counterions, for the derivatives in the order of free acid, NHS-ester, maleimide.
Structure: Structure of the free-acid derivative.
Spectra:

Spectra as determined in aqueous solution.

lAbs:

Longest-wavelength absorption maximum.
eMax Molar extinction coefficient at the longest-wavelength absorption maximum.
CF: CF280 = e280/emax. Correction factor used in the determination of degree of labeling (DOL) in case of dye-protein conjugates.
CF260 = e260/emax. Correction factor used in the determination of degree of labeling (DOL) in case of dye-DNA conjugates.
lFl :
Fluorescence maximum.
hFl: Fluorescence quantum yield.
tFl : Fluorescence decay time.

 

Impint

last revised 03/14/2007