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ATTO 594 is a new fluorescent label belonging to the class of Rhodamine dyes. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high thermal and photostability, excellent water solubility, and only little triplet formation.
ATTO 594 and its derivatives are supplied as crystalline, solvent-free solids. Due to particular substituents ATTO 594 is very hydrophilic. It shows excellent solubility in water, DMSO, DMF, as well as acetonitrile, and Chloroform. After coupling to a substrate the label carries a net electrical charge of -1. For details of coupling see our recommended labeling procedure (PDF). The fluorescence is excited most efficiently in the range 585 - 615 nm.


ATTO 594 - Optical Properties in Water

labs

601

nm

emax

1.2×105

l / mol cm

lfl

627

nm

hfl

85

%

tfl

3.5

ns

CF260 = e260/emax

0.26

 

CF280 = e280/emax

0.51

 

ATTO 594 is available

 with free carboxy group
 as NHS-ester
 as maleimide
 coupled to biotin

 

AD 594-2
AD 594-3
AD 594-4
AD 594-7

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Conjugates with phalloidin, avidin, streptavidin, BSA, etc. on request.

         

Impint

last revised 03/14/2007