ATTO 532 is a new fluorescent label related to the well-known dye Rhodamine 6G. Characteristic features of the label are strong absorption, high fluorescence quantum yield, high photostability, excellent water solubility, and very little triplet formation.

ATTO 532 is supplied as a crystalline, solvent-free solid. Due to particular substituents ATTO 532 is very hydrophilic. After coupling to a substrate the label carries a net electrical charge of -1. For details of coupling see our recommended labeling procedure (PDF). The fluorescence is excited most efficiently in the range 515 - 545 nm. A suitable excitation source for ATTO 532 is the 532 nm line of the frequency-doubled Nd:YAG laser.