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ATTO 488 is a new fluorescent label with excellent water solubility. The dye shows strong absorption, high fluorescence quantum yield, high photostability, and only little triplet formation. |
ATTO 488 is supplied as a crystalline, solvent-free solid. Due to particular substituents ATTO 488 is very hydrophilic. After coupling to a substrate the label carries a net electrical charge of -1. For details of coupling see our recommended labeling procedure (PDF). The fluorescence is excited most efficiently in the range 480 - 515 nm. For instance the 488 nm line of the Argon-Ion laser is very suitable for excitation. |
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ATTO 488 - Optical Properties in Water
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labs |
501 |
nm |
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emax |
9.0×104 |
l / mol cm |
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lfl |
523 |
nm |
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hfl |
80 |
% |
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tfl |
3.2 |
ns |
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CF260 = e260/emax |
0.25 |
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CF280 = e280/emax |
0.10 |
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ATTO 488 is available
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with free carboxy group
as NHS-ester
as maleimide
coupled to biotin
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AD 488-2
AD 488-3
AD 488-4
AD 488-7
Prices
Order |
Conjugates with phalloidin, avidin, streptavidin, BSA, etc. on request.
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