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ATTO 488 is a new fluorescent label with excellent water solubility. The dye shows strong absorption, high fluorescence quantum yield, high photostability, and only little triplet formation.
ATTO 488 is supplied as a crystalline, solvent-free solid. Due to particular substituents ATTO 488 is very hydrophilic. After coupling to a substrate the label carries a net electrical charge of -1. For details of coupling see our recommended labeling procedure (PDF). The fluorescence is excited most efficiently in the range 480 - 515 nm. For instance the 488 nm line of the Argon-Ion laser is very suitable for excitation.


ATTO 488 - Optical Properties in Water

labs

501

nm

emax

9.0×104

l / mol cm

lfl

523

nm

hfl

80

%

tfl

3.2

ns

CF260 = e260/emax

0.25

 

CF280 = e280/emax

0.10

 

ATTO 488 is available

 with free carboxy group
 as NHS-ester
 as maleimide
 coupled to biotin

 

AD 488-2
AD 488-3
AD 488-4
AD 488-7

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Conjugates with phalloidin, avidin, streptavidin, BSA, etc. on request.

         

Imprint

last revised 03/14/2007