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ATTO 425 is a new fluorescent label with a coumarin structure. Characteristic features of the label are high fluorescence quantum yield, large Stokes-Shift, and high stability within the physiological pH-range. The dye is moderately hydrophilic. Its NHS-ester and maleimide show excellent solubility in polar solvents like DMF, DMSO or acetonitrile. For details of coupling see our recommended labeling procedure (PDF). |
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ATTO 425 is supplied as a crystalline, solvent-free solid. Its fluorescence can be excited efficiently in the range 405 - 455 nm. |
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ATTO 425 - Optical Properties in Water
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labs |
436 |
nm |
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emax |
4.5×104 |
l / mol cm |
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lfl |
484 |
nm |
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hfl |
90 |
% |
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tfl |
3.5 |
ns |
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CF260 = e260/emax |
0.27 |
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CF280 = e280/emax |
0.23 |
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ATTO 425 is available
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with free carboxy group
as NHS-Ester
as maleimide
coupled to biotin
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AD 425-2
AD 425-3
AD 425-4
AD 425-7
Prices
Order
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Conjugates with phalloidin, avidin, streptavidin, BSA, etc. on request.
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