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ATTO 425 is a new fluorescent label with a coumarin structure. Characteristic features of the label are high fluorescence quantum yield, large Stokes-Shift, and high stability within the physiological pH-range. The dye is moderately hydrophilic. Its NHS-ester and maleimide show excellent solubility in polar solvents like DMF, DMSO or acetonitrile. For details of coupling see our recommended labeling procedure (PDF).
 
ATTO 425 is supplied as a crystalline, solvent-free solid. Its fluorescence can be excited efficiently in the range 405 - 455 nm.


ATTO 425 - Optical Properties in Water

labs

436

nm

emax

4.5×104

l / mol cm

lfl

484

nm

hfl

90

%

tfl

3.5

ns

CF260 = e260/emax

0.27

 

CF280 = e280/emax

0.23

 

ATTO 425 is available

 with free carboxy group
 as NHS-Ester
 as maleimide
 coupled to biotin

 

AD 425-2
AD 425-3
AD 425-4
AD 425-7

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Conjugates with phalloidin, avidin, streptavidin, BSA, etc. on request.

         

Imprint

last revised 01/15/2007

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