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ATTO 390 is a new fluorescent label with a coumarin structure. Characteristic features of the label are high fluorescence quantum yield, large Stokes-Shift and high stability at physiological pH-values. The dye is moderately hydrophilic. Its NHS-ester and maleimide show excellent solubility in polar solvents like DMF, DMSO or acetonitrile. For details of coupling see our recommended labeling procedure (PDF).
 
ATTO 390 is supplied as a crystalline, solvent-free solid. Its fluorescence can be excited efficiently in the range 360 - 410 nm. A useful excitation source is, e.g., a Mercury Arc Lamp with its lines at 365 nm and 405 nm.


ATTO 390 - Optical Properties in Water

labs

390

nm

emax

2.4×104

l / mol cm

lfl

479

nm

hfl

90

%

tfl

3.8

ns

CF260 = e260/emax

0.52

 

CF280 = e280/emax

0.08

 

ATTO 390 is available

 with free carboxy group
 as NHS-ester
 as maleimide
 coupled to biotin

 

AD 390-2
AD 390-3
AD 390-4
on request

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Conjugates with phalloidin, avidin, streptavidin, BSA, etc. on request.

         

Imprint

last revised 03/14/2007